Search results for " concentration" in Articles / App Notes
Article
Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
Downstream processing goals include multiple stages of purification and concentration. Disposable, single-use filtration is an alternative to outdated centrifugation methods that are difficult to scal…
Article
Single-use Bioreactors Have Reached the Big Time
Newer technologies have more recently been introduced for determination of viable cell mass, glucose, and lactate concentrations.
For instance, Sartorius’ Viamass sensor for online viable cell den…
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Evaluating E&L Studies for Single-Use Systems
It is important to highlight that in this type of extraction study design, if no dilution is applied from drug substance to drug product, the results in terms of concentration of the detected compound…
Article
Perfusion in the 21st Century
If specific productivities are kept high, higher cell concentrations (generally, greater than 100 x 106vc/mL) can be attained resulting in greater volumetric productivity (more than 1.5 g/L day). Incr…
Article
Make buffer preparation more time and cost efficient with inline conditioning
Simplify Buffer Preparation
The volume and number of buffers for a typical downstream process can be considerable.
Preparation of these buffers is a challenge that in-line conditioning can o…
Article
An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
Chemically defined raw materials have all components identified; components are in the chemical form, and the structure and concentration of all components is known and of high purity with only minima…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
…4), and the mutation rates measured by 6-thioguanine (6-TG) assay positively correlated with the MTX concentrations used to select the recombinant cell lines (3). Because multiple rounds of amplifica…
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Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
This method, widely named trypsinization, is based on the addition of an active concentration of the enzyme to the cell culture and subsequent digestion of cell-membrane proteins that establish the in…
Article
Robust Optimization, Simulation, and Effective Design Space
Figure 1: Robust optimization for a target concentration.
Step Seven: Optimize the process and define the set points
When determining the recipe for a formulation or process (set points) there…
Article
Biopharma Advances Demand Specialized Expertise
The main roadblock for continuous processing is downstream processing of proteins, which currently demands discrete and distinct unit operations for removal of impurities, removal of contaminants, con…