Search results for "chromatography"
Article
Moving PAT from Concept to Reality
In addition, a variety of on-line tools measure cell density (dielectric spectroscopy, fluorescence spectroscopy); protein aggregates (size-exclusion chromatography and ultra-high-performance liquid c…
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Biopharmaceutical Process Development: 2018 Lessons and 2019 Predictions
Readers also wanted to learn about specific solutions for downstream processing, with another hot article being an application note describing the development of downstream chromatography steps for th…
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Ensuring Viral Safety of Viral Vaccines and Vectors
These “viral clearance” or “viral removal” steps usually entail inactivation, chromatography, and/or virus filtration.
The fact that viral vaccines and vectors are actual viruses limits the ap…
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Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
Soluble aggregates and subvisible particle counts were measured by size-exclusion chromatography and micro-flow imaging, respectively. Thermal analysis of protein samples was performed by modulated di…
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Maximize resin usage with continuous bioprocessing
Periodic counter-current chromatography (PCC) is a multi-step approach to maximize the chromatography resin capacity utilization and minimize process time. The technology employs three or more chromat…
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Continuous Processing for the Production of Biopharmaceuticals
…sion, there has also been extensive development of other separation technologies such as continuous chromatography, refolding, precipitation, cell lysis, filtration, and aqueous two-phase extraction …
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Tackling Analytical Method Development for ADCs
Chromatographic methods based on hydrophobicity (hydrophobic interaction chromatography [HIC]) and size (size-exclusion chromatography [SEC], SEC-multi-angle laser light scattering (SEC-MALLS) can pro…
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Analyzing Proteins Using SEC, MALS, and UHPLC
Size exclusion chromatography (SEC) is generally used as a non-denaturing method that provides information on the apparent molecular weight, the presence of aggregates or higher molecular weight (MW) …
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Report from the 6th International HTPD Conference
HTPD was mainly limited to 96-well plates filled with chromatography resins used for resin selection and initial screening of running conditions. Since then, HTPD in chromatography applications has be…
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Biomanufacturing: Demand for Continuous Bioprocessing Increasing
Multi-column, countercurrent, and other variations of continuous chromatography units are just starting to enter the market. The classic and still predominant approach to bioprocessing, both upstream …