Search results for "chromatography"
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Developing an Efficient Adenovirus Downstream Purification Process
This application note describes the development of downstream chromatography steps for purification of adenovirus from HEK293 cell culture harvest.
To read the application note click her…
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Gain Efficiency in Process Development
The development of efficient manufacturing processes is a necessity for cost-effective production of biopharmaceuticals. By carefully designing and evaluating your experiments, sufficient informatio…
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Your Protein Purification Questions Discussed
Questions about chromatography techniques, troubleshooting tips and tricks, and the use of ÄKTA systems were submitted in writing by protein purifiers globally.
We have included the questio…
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Tools and Solutions for Separation of Charged mAb Variants
A biosimilar is an almost identical version of an originator product. To attain regulatory approval, a comparable quality to the reference product in terms of efficacy, purity, and safety should be …
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Handbook: Design of Experiments in Protein Production and Purification
Design of experiments (DoE) is a technique for planning experiments and analyzing the information obtained. The technique allows us to use a minimum number of experiments, in which we systematically v…
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The New World of Biopharmaceutical Manufacturing
Single-use sensors have been developed for a variety of process parameters for downstream steps such as chromatography, tangential flow filtration, direct filtration, and fill/finish, as well as for s…
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Column Tips for Successful SEC-MALS
Do you identify molecules using MALS, maybe in combination with size exclusion chromatography (SEC)? We have gathered Column Tips for Successful SEC-MALS in a blog post. Tap into these column …
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Extractables Studies for Single Use Systems Used in ADC Manufacturing, Part 1
To address those concerns, extractables studies were
performed on disposable chromatography column housings and disposable flow paths provided by Cytiva. The extractables studies were performed wi…
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Being Thorough When Transferring Technology
[In regards to chromatography steps], sometimes the linear flow needs to be lowered due to the volumetric flow restrictions in the large-scale facility. This would have to be tested during process opt…
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Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
Traditional manufacturing processes for biologics make use of large, fed-batch reactors and oversized chromatography columns that end up wasting time or material. Alternatively, upstream process scale…