Search results for " Protein A"
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Preclinical Evaluation of Product Related Impurities and Variants
For example, the cystine/DTT ratio is governed by the number of cysteine residues in the protein and thus the number of disulfide bonds necessary for the protein to assume its functional native confor…
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ADC Development Robust Despite Lackluster Performance
Instead, the company’s approach is to use the protein as a cancer-associated-fibroblast-specific vehicle to internalize cytotoxic molecules because MTX5 is naturally capable of internalizing molecules…
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From Darwin to Recombinant Fc Multimers
Topics from a Darwinistic viewpoint on how business models are evolving in the Life Science industry to breakthrough research data from Momenta Pharmaceuticals on a recombinant trivalent Fc protein …
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Being Thorough When Transferring Technology
Communication and taking the time to develop the process are key to successful transfer and scale up of biologics.
By Susan Haigney
Editor's note: The following is an extended version of the…
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Affinity Chromatography Handbook in 3 Volumes
Affinity chromatography (AC) separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The techni…
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Optimizing Resin Performance with Disposable Chromatography Solutions
Where we have seen significant advances in chromatography is with innovative new Protein A-derived media that are delivering improved capacity and stability, helping to increase production efficiency,…
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As the therapeutic pipeline diversifies, chromatography toolboxes are expanding.
For traditional mAbs, protein A chromatography is used to capture antibodies. Depending on the structure of the target antibody and the impurity profile, other affinities, like protein L or variants o…
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Choose your Optimal Wavelength for Chromatography
U9-T monitor allows differentiation between peaks containing protein and those containing DNA/RNA, nucleic acids or nucleotides. Most proteins absorb strongly at 280 nm due to the presence of tryptoph…
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Chromatography for the Diversified Antibody Pipeline
We can see the protein and understand the necessity for having other tools targeting regions on the antibodies such as Kappa ligand or the Lambda ligand. These were developed for fragments and not alk…