Search results for " Protein A" in Articles / App Notes
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Address the complexity of protein characterization
Since the FDA approved the first monoclonal based therapy in 1985, mAbs have had significant clinical success and have been used to provide effective treatment for a range of diseases. Durin…
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Current Trends in Host Cell Protein Detection for Biologics Manufacturing
Commonly accepted HCP analytical methods, the challenges of HCP assay, and recommendations on what can easily be accomplished in-house and when it may be better to outsource.
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Optimization of a HIC step for recombinant protein purification
Follow this step-by-step high-throughput process development (HTPD) workflow to determine the optimal resin and initial conditions for hydrophobic interaction chromatography (HIC).
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Videos! SPR Applications in Protein Characterization
In these exclusive videos from the Biacore Analytical, Comparability and QC Symposium, Vienna, November 2017, industry thought-leaders share insights, tips, and techniques that illuminate how SPR with…
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Get Acquainted with How SPR Technology is Used for Better Protein Characterization
Surface plasmon resonance (SPR) has become a go-to tool for biotherapeutic characterization, and most approved antibody therapeutics have passed through a Biacore SPR system.
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Addressing the Challenges in Downstream Processing Today and Tomorrow
The cost of the virus-retaining filter, which can approach that of the protein A resin, can be reduced by using prefilters or optimizing protein concentration, temperature, buffer composition, and sol…
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Impurity Testing of Biologic Drug Products
“Product-related impurities can be categorized as product variants, and basically correspond to any undesired modification of the protein amino-acid sequence or post-translational modifications,” she …
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Impact of Media Components on CQAs of Monoclonal Antibodies
Cysteine, along with temperature shift, is believed to reduce protein aggregates and increase their stability in culture harvests. The oxidized form of cysteine has been shown to reduce the formation …
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Modeling the Degradation of mAb Therapeutics
Mathematical modeling of protein aggregation correlated along with kinetic data can help developers gain both qualitative and quantitative insights into the mechanism behind aggregation. Armed with th…
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Generating a Fully Processed Antibody
The authors outline cell-line development and process scale-up for an antibody program in which the antibody requires additional processing by a site-specific enzyme for correct functionality.
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