Search results for " buffer"
Poster
Scale-up and process economy calculations of a dAb purification process using ready-to-use products
In addition, process economy of using ready-to-use equipment and ready-made buffers versus using reusable equipment and in-house prepared buffers was compared. A three-step dAb purification process wa…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
The cost of the virus-retaining filter, which can approach that of the protein A resin, can be reduced by using prefilters or optimizing protein concentration, temperature, buffer composition, and sol…
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Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Over time, the buffer replaces the analyte and the dissociation rate of the analyte is monitored. Both the association and dissociation curve can be fitted to one of the chosen models. In addition, th…
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The Challenge of Disruptive Technologies in Bioprocessing
“Market trends toward efficiency, single-use bioprocessing, flexible facilities, and increased outsourcing of non-core activities are driving considerations for buffer outsourcing. Customers are deman…
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Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
MATERIALS AND METHODS
Monoclonal antibody (mAb-A, IgG1) formulation
The mAb-A formulation (64 mg/mL protein concentration) was prepared in a formulation that contained a buffering agent (10 mM…
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Biopharmaceutical Process Development: 2018 Lessons and 2019 Predictions
One hot article answered two commonly asked questions about in-line buffer preparation, which were “Will our buffers work?” and “How can we be convinced that buffers are made to specification?”
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Driving Change in Biomanufacturing
The large buffer volumes required for biomanufacturing can be a bottleneck, especially when scaling up. Just-in-time buffer preparation can be the solution. In this blog, we discuss the two most commo…
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Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
Next, if known, specify the buffer and media requirements of each unit operation step in the production process, including whether any solutions require special handling (e.g., 70% ethanol), if steps …
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Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The properties of octylamine ligand, allowing for binding of impurities over a broad pH range, high salt concentrations, and in various buffer compositions, enable the use of Capto Core 700 chromatogr…
Article
Connected Inline Conditioning for Better Process Efficiency
This automated the buffer preparation process, saving time, space, and costs.
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