Search results for " ion-exchange"
Article
Improving Process-Scale Chromatography
Developments are also being made in ion-exchange chromatography, primarily in the area of capacity improvements to reduce cost and improve ease of use, according to Lynch. Thermo Fisher has continued …
Article
Downstream Processing Continues to Worry CMOs
CMOs are working hard to improve performance by investigating new technologies for filtration and purification.
By Eric S. Langer
Downstream processing continues to be a significant pain poi…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The highest DBC on an ion-exchange chromatography media is typically expected at low conductivity and at a pH far away from the isoelectric point (pI), where the protein charge is high. For some prote…
Article
Host-Cell Protein Measurement and Control
With an additional separation dimension by high pH reversed phase, ion-exchange, or size-exclusion before LC-MS/MS identification, 2D-LC-MS reduces the interference from dominant peptides digested fro…
Article
SEC in the Modern Downstream Purification Process
Biomolecules typically should not bind to an SEC resin like they do in ion-exchange chromatography, affinity chromatography, hydrophobic interaction, or mixed-mode chromatography. As a result, mobile-…
Article
Mechanistic Modeling: Does it Have a Future in Process Development?
For some unit operations, such as ion-exchange chromatography, mechanistic models are easily employed for prediction. However, when it comes to reverse-phase chromatography or HIC, the mathematical de…
Article
Challenges of Protein Aggregation During Purification
Careful selection of downstream processing conditions is a must.
Sep 1, 2014
By: Cynthia Challener, PhD
BioPharm International
Volume 27, Issue 9, pp. 32-35
Removal of protein a…
Article
2014 Manufacturing Trends and Outlook
Single-use systems and other technologies drive process efficiencies, but there is room for improvement.
In a recent survey of current trends and practices in biopharmaceutical manufacturing…
Article
QbD and PAT in Upstream and Downstream Processing
For chromatography steps, these may include protein load, pH (load or elution depending on step), temperature, flow rate, and conductivity for ion-exchange steps. Viral inactivation steps may have tem…
Article
Quality by design for biotechnology products—part 1
Examples include:
analytical tools such as native isoelectric focusing (IEF), ion-exchange chromatography (IEC), and online mass spectrometry to quantify the level of deamidation
preclin…