Search results for " ion-exchange" in Articles / App Notes
Article
Mechanistic Modeling of Preparative Ion-Exchange Chromatography
For example, for the commonly used ion-exchange chromatography, pH, ion concentration, gradient, and stationary phase can affect the recovery step as well as the resulting product quality.
For com…
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Asked and Answered: Your Questions on Ion-Exchange Chromatography
Check out what questions your fellow protein purifiers have related to ion-exchange chromatography.
The questions are organized in by chief concern, and include buffers, experimental design, L…
Article
Process Chromatography Selection for Downstream Processing Applications
For these molecules, chromatographic techniques such as ion-exchange, hydrophobic interaction chromatography, and multimodal resins are common. Affinity resins like Capto L (antigen-binding fragments …
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Multi-product high-resolution monoclonal antibody charge variant separations by pH gradient ion-exchange chromatography. Anal Chem. 2009;81:8846–57.
17. Salas-Solano O, Gennaro L, Felten C. Optimi…
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CMOs Continue to Improve Overall Biomanufacturing Performance
Many are also using or evaluating a number of technologies, including:
• Membrane-based filtration technologies
• Ion-exchange membrane technologies
• Ion-exchange technologi…
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Evolution of the Monoclonal Antibody Purification Platform
Use of multimodal chromatography
Recently, mixed-mode, ion-exchange ligands with enhanced binding strength for aggregates through the addition of hydrophobic functionality have been brought to mar…
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Trends in Downstream Bioprocessing
Downstream processing involves the recovery and purification of protein therapeutics for biopharmaceuticals, vaccines, and other biologics. It involves the removal of insoluble cell debris and parti…
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How Can A Chromatography Bead Free Up Your Time?
Have you ever considered how a resin bead in your chromatography column can affect your ion exchange (IEX) chromatography run time?
Read this blog and learn how you can optimize the time spen…
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Impurity Testing of Biologic Drug Products
… of cellular origin via techniques such as SEC–HPLC, hydrophobic interaction chromatography (HIC), ion-exchange HPLC, and isoelectric focusing (IEF) capillary electrophoresis. Process-related impurit…
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Process Chromatography: Continuous Optimization
Several interaction modes
There are four major chromatographic techniques employed to purify biologic drug substances: affinity, ion-exchange (IEX), mixed- or multi-mode, and hydrophobic-interaction…