Search results for " polishing" in Articles / App Notes
Article
Improving Process-Scale Chromatography
Pall recently introduced a cation-exchange mixed mode sorbent that is effective in post-Protein A mAb polishing when used in conjunction with membrane chromatography in the flow-through mode.
Cyti…
Article
Process Chromatography Selection for Downstream Processing Applications
The use of activated carbon as an adsorbent in polishing steps is gaining attention in the industry. This technology can provide good host cell protein removal within a relatively broad operating wind…
Article
Cleaning of Dedicated Equipment: Why Validation is Needed
This article discusses cleaning validation of equipment dedicated to the production of a single API.
By Cristina Baccarelli, Paola Bernard, Teresa Cortellino, Oscar Cruciani, Rita Pacello, Chi…
Article
High-Throughput Process Development in an Historical Environment
Two different novel multimodal polishing Capto™ ImpRes resins were evaluated and the optimal ligand density was identified to provide best aggregate clearance and largest operating space.
In addit…
Article
Challenges of Protein Aggregation During Purification
Hydrophobic interaction chromatography is generally employed as a polishing step for removing impurities based on hydrophobicity. At the same time, strong hydrophobic interactions between the resin an…
Article
Q&As with Industry Leaders
… Northrop, BioPharm International
Jakob Liderfelt, Global Product Manager, Monoclonal Antibody Polishing, Cytiva
Top challenges in recombinant protein purification process development…
Article
Trends in Downstream Bioprocessing
…product purification through affinity chromatography, crystallization, or precipitation; and product polishing and virus removal.
Downstream processing (DSP) has seen significant challenges over …
Article
A Platform Approach for the Purification of Domain Antibodies (Dabs)
This Application note describes a three-step purification process of a domain antibody (Dab) expressed in the periplasm of E. coli. First, a capture step using Capto™ L was used to reduce E. coli host…