Search results for " polishing" in Articles / App Notes
Article
A deep dive into optimizing AAV capture and polishing to maximize both yield and purity
To meet these needs, Cytiva developed scalable capture and polishing chromatography steps for recombinant AAV (rAAV). The affinity capture step maximizes recovery and efficiently removes impurities.
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Article
Guide to Polishing Chromatography in Process Development
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This article shares the factors you should consider when developing your polishing steps—from the basics to the typical challenges faced, and how to overcome them.
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Article
HTPD: Optimizing a mAb Chromatography Polishing Step
In this webinar, a case study using high throughput process development tools, optimizing process conditions for a mAb polishing step using a multimodal chromatography resin (Capto MMC ImpRes), will b…
Article
Technical and Process Economical Aspects of Using Capto™ Q and ReadyToProcess™Adsorber Q in mAb Polishing
Here, we compare the performance of the disposable ReadyToProcess Adsorber Q membrane capsule with Capto Q resin packed in conventional chromatography columns when used in the final polishing step of …
Article
Dear lentivirus: We have not forgotten about you and how to improve your polishing recovery
Lentiviruses are commonly used for both chimeric antigen receptor (CAR) T cell therapy and ex vivo gene therapy.
Vector purification is very challenging due to low stability of these envel…
Article
Developing a HIC polishing step for removal of mAb aggregates
This article describes the development of a flow-through (FT) polishing step using hydrophobic interaction chromatography (HIC) to remove mAb aggregates.
It includes:
- The use of HTPD plate…
Article
Optimization of an antibody polishing step
Antibody purification processes are typically developed using a platform approach. Optimal operating conditions for single units are found by altering just a few process parameters with De…
Article
Connected Polishing and Concentration Under One Automation Method
Here, the ÄKTA™ ready chromatography system was directly connected to the ÄKTA readyflux filtration system, for polishing and concentration of a target molecule. One automation method was cr…
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Optimizing capture and polishing steps in an rAAV purification process
In this article, we show how we developed an efficient purification process for recombinant AAV (rAAV). The chromatography purification described includes affinity capture to maxi…
Article
Development of Purification for Challenging Fc-Fusion Proteins
…els, and a novel virus-spiking strategy, the authors have developed an alternative Fc-fusion protein polishing process that incorporates flow-through strategy in polishing steps using cation exchange…