Search results for " medium"
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
These methods primarily target the cell-culture medium before its transfer to the bioreactor as well as raw materials of animal or human origin. Options include high temperature/short time (HTST), C s…
Article
Evaluating E&L Studies for Single-Use Systems
The risk ratings of each factor will be weighted as in the BPOG example and summed to obtain the final risk score that is then categorized as low, medium, or high. If the risk is classified as low, it…
Article
Perfusion in the 21st Century
With perfusion technology, fresh medium is continuously introduced while spent medium, which generally contains the API and waste products, is removed. Cells are retained in the bioreactor by a device…
Article
Maximum Output Starts with Optimized Upstream Processing
On a positive note, parallelization of experiments using automated systems for high-producer clone selection, along with medium optimization and the availability of standard kits, are having an overwh…
Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
…ctor being manufactured, the type of cells used, and whether the virus is harvested from the culture medium, the cells, or both. The single-use systems most used, according to Chor Sing Tan, strategy…
Article
Gene Therapies Push Viral Vector Production
In addition, the growth factors need to be continuously and manually added/replenished in the medium. This cumbersome process leads to an increase in the chances of errors.
• Cell expansion …
Article
Best Practices for Selecting a Top-Quality Cell Line
The plasmid ratio and medium are also important, Cai observes. “A balanced plasmid ratio usually has dramatic impact on some formats of bispecific antibody cell line development to minimize impurities…
Article
Optimization of Fed‑Batch Culture Conditions for a mAb‑Producing CHO Cell Line
In the first step, optimal feed combinations were screened by spiking the basal medium with different Cell Boost combination on day 0 of batch cultures. The selected Cell Boost supplements were therea…
Article
Challenges in Cell Harvesting Prompt Enhancements
Cell harvesting involves the separation of the cell-culture from the medium and running it through several techniques: centrifugation, microfiltration, depth filtration, and filtration through absolut…
Article
Generating a Fully Processed Antibody
Twenty-four clones were selected for further evaluation, eight with high FGE gene copy, eight with medium, and eight with low. The 24 clones were grown in culture for five weeks, and FGE level was ana…