Search results for " medium"
Article
Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
Sample preparation was performed by liquid-liquid extraction with dichloromethane at below pH 3, above pH 11, and with no pH adjustment prior to analysis of cell-culture medium incubated in single-use…
Article
Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
The bags were exposed from 1 to 3 cycles (1x-3x) of either fast freeze/fast thaw, fast freeze/slow thaw, medium freeze/medium thaw, slow freeze/fast thaw, or slow freeze/slow thaw. A liquid control wa…
Article
Optimization of Protein Expression in Escherichia Coli
There are many parameters that affect cell growth and recombinant protein expression, such as choice of medium, carbon source, temperature, pH, aeration, inducer concentrations, and length of inductio…
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
A combined approach of NIR and MVDA was used for the identification and qualification of basal medium powder components (9) and was evaluated as an identity tool for cell-culture media (10).
Raman…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
…d the initial mAb capture step was carried out with an alkali-stabilized, Protein A-derived affinity medium using a standard protocol. An HTPD approach was then taken for the subsequent purification …
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
A common challenge upon cell transfer is the lag phase, during which the cells are adjusting to the new culture medium and little or no increase in cell number is observed. In the 50 L cultivations, t…
Article
Commercial Production of Gene Therapies Using Suspension or Adherent Cell Lines
By comparison to STRs, FBRs are “segregated” systems where the cells, immobilized in the bed, are decoupled from the liquid medium and regulations, which allows for parameters to be optimized with les…
Poster
Benchmarking, scalability, and protein production in CHO cell culture
With the large number of CHO clones in use, it is important to have a cell culture medium that supports high viable cell counts and productivity with multiple cell clones. A precise balance of nutri…
Article
Determining Criticality-Process Parameters and Quality Attributes Part I: Criticality as a Continuum
For Stage 1, however, often a qualitative risk assessment evaluating low, medium, and high risk is sufficient to distinguish relative differences in risk.
Continuum of CQAs
Prior to the develo…
Article
The use of Xcellerex™ mixing system as slurry tank when packing chromatography columns
The capability of the mixing system to keep the chromatography medium (resin) in suspension and the possibility to integrate the system in column packing were studied. The results show that XDM Quad …