Search results for " capture"
Article
A deep dive into optimizing AAV capture and polishing to maximize both yield and purity
To meet these needs, Cytiva developed scalable capture and polishing chromatography steps for recombinant AAV (rAAV). The affinity capture step maximizes recovery and efficiently removes impurities.
…
Webcast
Strategies and emerging technologies for mAb capture
Strategies will depend on the mode of upstream operations (fed batch or perfusion) and the desired process improvement of the capture step, e.g., productivity, cost, or process time, as well as the ty…
Article
Strategies and Emerging Technologies for mAb Capture
Strategies will depend on the mode of upstream operations (fed batch or perfusion) and the desired process improvement of the capture step, (e.g., productivity, cost, or process time) as well as the t…
Article
Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecif…
Article
Optimizing capture and polishing steps in an rAAV purification process
The chromatography purification described includes affinity capture to maximize recovery with efficient impurity removal.
READ FULL ARTICLE >>
Article
Capture of Bispecific Antibodies and Removal of Product-Related Impurities
One potential solution is to initiate polishing at the capture step using differences in avidity. In this article, Cytiva presents data on applications using MabSelect™ VL protein L resin with novel …
Article
Case Study: Virus Reduction of an Affinity Capture Step
In this study, the virus reduction of an affinity capture step was evaluated in a scale-down column format. The study was performed by a third-party viral testing laboratory in collaboration with a bi…
Article
Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
In this work, a multidimensional scale-up (change of both column diameter and bed height) of a mAb capture step, using the ÄKTA pilot 600 chromatography system, is demonstrated. Column volume per…
Resource
Strategies and Emerging Technologies for mAb Capture
20 May 17 CEST | 11 EDT
Article
Process Economy Simulations on a Protein A Capture Step
MabSelect PrismA is a next‐generation protein A chromatography resin that offers significantly enhanced alkaline stability and capacity compared with its predecessor products. This application note …