Search results for " capture" in Posters
Poster
A Next Generation Protein A Chromatography Resin for Improved Productivity and Bioburden Control
• The increased upstream titers in mAb production seen in recent years may make the Protein A capture step a bottleneck.
• Protein A columns columns are also more prone to bioburden contamination…
Poster
Scalable Continuous Chromatography Process for Enhanced Efficiency in Biomanufacturing
… that PCC is a robust and scalable technology: a 50-fold scale-up of a three-column PCC (3C PCC) mAb capture step within the lab-scale ÄKTA™ pcc system and a 294-fold scale-up directly from lab …
Poster
Scale-up and process economy calculations of a dAb purification process using ready-to-use products
Purification of a domain antibody (dAb) in a chromatography capture step was successfully scaled from laboratory to pilot scale using ready-to-use equipment and ready-made buffers. The scaled-up pro…
Poster
Residual DNA analysis in influenza vaccine processing
In capture mode, recoveries of 40%–60% were achieved after elution. However, recovery could be improved by concentration determination of DNA bound to the resin.
Poster
Case Study: Improve Process Economy by Cycling of Prepacked Chromatography Columns
As projects are scaling up to manufacturing scale, it becomes attractive to cycle equipment for specific campaigns for full capture of process economy gains. This customer case study evaluates proce…
Poster
Fast Trak Services: a collaborative project to accelerate downstream biosimilar process development
Process optimization focused on improving the affinity chromatography (AF) capture step as well as the intermediate purification and polishing steps, using hydrophobic interaction chromatography (HIC…
Poster
Evaluation of Protein A resin lifetime during extensive use (overloading) in continuous chromatography mode
This application note compares the performance of MabSelect SuRe pcc resin designed for continuous capture of mAbs, with the conventional high-capacity Protein A resin MabSelect SuRe LX. The study was…
Poster
Guide to modern BioProcess™ chromatography resins
Many bioprocess applications include three chromatography steps: capture to isolate, concentrate, and stabilize the protein. There is an intermediate step where bulk impurities are removed followed by…
Poster
Optimizing productivity on high capacity protein A affinity medium
MabSelect SuRe™ protein A affinity medium has long been considered a starndard tool for capturing monoclonal antibodies in biopharmaceutical production. This application note summarizes studies on d…
Poster
Purification platform challenges and strategies for handling of "tricky" MAbs
In the work presented here, capture using high-capacity protein A medium (resin) was followed by polishing using a novel cation exchange chromatography medium.