Search results for " qPCR"
Article
Emerging Therapies Test Existing Bioanalytical Methods
Specialty assays used to understand the distribution, function, efficacy, and immunogenicity of gene- and cell-therapy drugs—including cell-based neutralizing assays, qPCR, flow cytometry and enzyme-l…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
FHD=first human dose, FRD=first registration dose, PCR=polymerase chain reaction, qPCR=quantitative polymerase chain reaction, MCB=master cell bank, RT-PCR=reverse transcription polymerase chain react…
Article
Concentrating Feed—an Applicable Approach to Improve Antibody Production
Cell counts, antibody titer, protein quality analysis, and qPCR array. Cell-culture samples were counted by a Vi-CELL (Beckman-Coulter) using the trypan blue exclusion method. The pH and metabolites i…
Article
Managing Residual Impurities During Downstream Processing
For DNA detection, Atouf notes that qPCR was included because it is by far the most commonly used technique in the industry due to its high sensitivity, specificity, dynamic range, high-throughput cap…
Article
COVID-19 Diagnostics Supplier Ramps Up Production with Help of Cytiva’s Testa Center in Sweden
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In this blog post, you can learn about how Solis BioDyne, Estonian manufacturer of polymerase chain reaction/quantitative polymerase chain reaction reagents to analyze DNA f…
Article
Impurity Testing of Biologic Drug Products
Experts share insights on the various methods used for purity and impurity analysis of therapeutic proteins.
By Adeline Siew, PhD
CA-SSIS/shutterstock.comIm…
Article
Development of Purification for Challenging Fc-Fusion Proteins
Development of Purification for Challenging Fc-Fusion Proteins
This study outlines methods for an alternative protein-polishing process for challenging proteins.
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Poster
Residual DNA analysis in influenza vaccine processing
In cell-based influenza vaccine production, the European Pharmacopoeia demands a host cell residual DNA concentration of less than 10 ng per dose. To reliably measure residual DNA in both process sa…