Search results for "purification"
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Enhancing Resins Addresses Purification Concerns
Resins have been seen as one tool that can address the purification needs of new COVID-19 vaccines as well as the existing challenges in the downstream processing of increasingly complex biologic moda…
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The Development and Application of a Monoclonal Antibody Purification Platform
The following article describes the development of a purification platform for monoclonal antibodies (MAbs) at Pfizer's Global Biologics division, including how the individual unit operations have bee…
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Comparing Protein A Resins for Monoclonal Antibody Purification
As a consequence, downstream processing must accept and handle higher titers of mAbs in harvested cell-culture fluid (HCCF), and vendors of mAb purification technologies must develop chromatography re…
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Development of Purification for Challenging Fc-Fusion Proteins
Monoclonal antibody purification processes exist in different well-established platforms with extensive process performance histories for production of commercial mAbs (6-10), which typically consist …
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Challenges of Protein Aggregation During Purification
Importantly, the conditions that can lead to aggregation can occur during purification processes and need to be avoided or mitigated. “In particular, resolubilization of insoluble aggregates typically…
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Evolution of the Monoclonal Antibody Purification Platform
This 31st article in the “Elements of Biopharmaceutical Production” series focuses on evolution of the purification platform for manufacturing of mAb therapeutics.
Traditional Mon…
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SEC in the Modern Downstream Purification Process
While SEC may have mostly been abandoned in monoclonal antibody (mAb) and other protein purifications, it is still commonly used in the purification of viruses and plasmids.
SEC, also known as gel…
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Top challenges in recombinant protein purification process development
I'm really looking forward to this discussion today on recombinant protein purification process development.
In the industry, we're seeing recombinant proteins more often, right? And we're seeing …
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Combining Purification Techniques in a Multistep Approach
Setting up a multistep purification might be the way forward for your lab.
With the right combination of different purification techniques, resins used, and how your methods are set up for a multi…
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Simplify Every Step of Histidine-Tagged Protein Purification
To improve this, an automated purification approach was evaluated. Learn more in this blog post how automated histidine-tagged protein purification can simplify your protein purification.