Search results for " binding"
Article
Best Practices for Selecting a Top-Quality Cell Line
Irina Shi - Stock.Adobe.com
Leveraging automation and a step-by-step approach are keys to success.
By Cynthia A. Challener
Cell lines determine the performanc…
Article
Overcome Chromatography Challenges with Fiber Adsorbents
This structure allows high mAb binding capacities at very short residence times. As a result, cycle times take minutes compared to the hours needed for resin-based chromatography.
In this article,…
Article
Driving Improved Access to Biosimilars
Driving Improved Access to Biosimilars
New guidance from FDA, including standards for interchangeability, is expected to speed development of biosimilars, but experts warn against oversimplifying ri…
Article
Moving PAT from Concept to Reality
…uminescent proximity homogeneous assay, and fluorescence resonance energy transfer for target and Fc binding analysis; liquid chromatography coupled with ultraviolet, fluorescence, or MS detectors fo…
Article
Western Blot Detection: Is Film or a Digital CCD Imager Better?
It is based on building an antibody:protein complex via specific binding of antibodies to proteins immobilized on a membrane and detecting the bound antibody with one of several detection methods.
…
Article
An Analytical Approach to Biosimilar Drug Development
… glycan profiles; the potency or biological activities; and other functional tests, such as receptor binding.
Analytical similarity by itself is not designed to ‘ensure’ biosimilarity, but is…
Article
Biopharmaceutical Process Development: 2018 Lessons and 2019 Predictions
…ure Conditions for a mAb‑Producing CHO Cell Line
• How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
Article
Scaling Up Novel Therapies
One example Ransohoff cites is plate-based chromatography resin screening, enabled by technologies such as Cytiva Predictor plates, that allow users to consider binding properties of resins and soluti…
Article
Capacity and Performance of a Next Generation Protein A Chromatography Resin
This application note demonstrates the binding capacity and purification performance of MabSelect PrismA in comparison with its predecessor products for both polyclonal and monoclonal antibodies. An i…
Article
Making the Move to Continuous Chromatography
Making the Move to Continuous Chromatography
Early adopters are benefiting from lower costs and increased productivity.
By Cynthia A. Challener
Tashatuvango/Shu…