Search results for " binding" in Articles / App Notes
Article
Advances in Engineering of Protein-Based APIs
“Various strategies have been developed recently to eliminate Fc-associated effector binding and functions such as antibody-dependent cell-mediated cytotoxicity and complement dependent cytotoxicity a…
Article
Fusion Proteins Pose Manufacturability Challenges
“These molecules are of interest because the domains may have functions that, when combined, are uniquely useful as therapeutics, such as binding/inhibition of cytokines and a longer half-life,” expla…
Article
Witnessing Major Growth in Next-Generation Antibodies
He notes that bispecific and multispecific antibodies have also been broadly applied in oncology for the neutralization of multiple antigens, the fusion of binding proteins to antibodies, and cell rec…
Article
Host-Cell Protein Measurement and Control
Jun 01, 2015
By Fengqiang Wang, PhD, Daisy Richardson, Mohammed Shameem
BioPharm International
Volume 28, Issue 6, pg 32–38
Host-cell proteins (HCPs) constitute a major part of proce…
Article
SEC in the Modern Downstream Purification Process
Mar 01, 2015
By R. Christopher Manzari, J. Kevin O'Donnell
BioPharm International
Volume 3, Issue 28
Since the introduction of commercial chromatography resins approximately six de…
Article
Optimizing Resin Performance with Disposable Chromatography Solutions
Highly porous resins tend to have a higher binding capacity since there is less steric hindrance caused by molecules bound to the substrate. Of course, there is a limit to porosity since resins must a…
Article
Address the complexity of protein characterization
This presentation includes some examples on the use of Biacore™ surface plasmon resonance (SPR) systems for active concentration measurement, target binding, and describe how you can use these assays …
Article
Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecif…
Article
Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
In this study, we compared two approaches for purifying a recombinant protein that does not have an affinity binding partner.
The first method is using nonaffinity chromatography steps (AI…
Article
Get Started: Purification of mAb Variants - eLearning
These molecules show wider variation in binding affinities and are often more challenging to separate from impurities, such as host cell proteins (HCP), host DNA, aggregates, and isoforms.
In this …