Search results for " CHO"
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Given this inherent genetic flux, recombinant genes (or transgenes, also referred to as the expression construct) expressed in CHO cells—used for therapeutic bioproduct development—can be subject to g…
Poster
Benchmarking, scalability, and protein production in CHO cell culture
With the large number of CHO clones in use, it is important to have a cell culture medium that supports high viable cell counts and productivity with multiple cell clones. A precise balance of nutri…
Article
Optimization of Fed‑Batch Culture Conditions for a mAb‑Producing CHO Cell Line
This application note describes a broadly applicable and efficient workflow toward finding the optimal feed combination for the best-performing fed-batch processes.
An IgG1-producing CHO cell …
Poster
Development of DoE based fed-batch strategies for high-producing CHO cell cultures
Fed-batch culture is commonly employed to maximize cell and product concentrations in upstream mammalian cell culture processes. Typical standard platform processes rely on fixed-volume bolus feedin…
Poster
Effects of feeding strategy on CHO cell performance in fed-batch cultures
ActiPro cell culture production medium is intended to be used in combination with Cell Boost 7a and 7b supplements to enhance recombinant protein production in fed-batch or perfusion processes. This…
Poster
Scale-up of CHO fed-batch cultures in HyClone™ ActiPro™ medium supplemented with Cell Boost™ 7a and 7b
Comparing cell growth and recombinant protein production of multiple Chinese hamster ovary (CHO) cell clones when cultured in HyClone ActiPro basal medium and Cell Boost 7a and 7b feeding supplement…
Poster
Repurposing Fed-Batch Media and Feeds for Highly Productive CHO Cell Perfusion Processes
The authors developed a method for identifying first-generation perfusion culture media based on existing fed-batch media and feeds. Here, they show that they can obtain culture media that successfull…
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
The choice of cell culture media has been known to significantly affect the physiochemical characteristics of mAbs. The selection of the media depends on the type of cells to be cultured and also the …
Article
Platform Technologies Improve Protein Expression
…sicians, and ultimately patients in need of novel medications,” notes Bjørn Voldborg, director of CHO cell-line development at the Novo Nordisk Foundation Center for Biosustainability.
Improvin…
Article
Generating a Fully Processed Antibody
Cell-line engineering for high-level protein expression and proper enzyme conversion
The goal of this case study was two-fold: to generate a stable CHO cell line expressing consistent high levels …