Search results for " CHO" in Articles / App Notes
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Given this inherent genetic flux, recombinant genes (or transgenes, also referred to as the expression construct) expressed in CHO cells—used for therapeutic bioproduct development—can be subject to g…
Article
Optimization of Fed‑Batch Culture Conditions for a mAb‑Producing CHO Cell Line
This application note describes a broadly applicable and efficient workflow toward finding the optimal feed combination for the best-performing fed-batch processes.
An IgG1-producing CHO cell …
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
The choice of cell culture media has been known to significantly affect the physiochemical characteristics of mAbs. The selection of the media depends on the type of cells to be cultured and also the …
Article
Platform Technologies Improve Protein Expression
…sicians, and ultimately patients in need of novel medications,” notes Bjørn Voldborg, director of CHO cell-line development at the Novo Nordisk Foundation Center for Biosustainability.
Improvin…
Article
Generating a Fully Processed Antibody
Cell-line engineering for high-level protein expression and proper enzyme conversion
The goal of this case study was two-fold: to generate a stable CHO cell line expressing consistent high levels …
Article
Platform Approach Speeds Process Development
An expression platform has demonstrated fast-track development of high-quality, high-titer processes for producing biopharmaceuticals from CHO cells. The platform includes vector design, cell line eng…
Article
N-Glycan Analysis of Biotherapeutic Proteins
…a and ADCC activity (15), contrasting with the lower activity of mostly fucosylated mAbs produced in CHO cells. Consequently, glycoengineereing approaches have been developed to generate afucosyl ant…
Article
State of the Biopharmaceutical Industry: Lessons from 2018 Survey Data
Despite diverse genetic-engineering methods and host organisms being available, industry has clearly come to prefer and standardize on use of mammalian systems, particularly CHO. Many more efficient o…
Article
Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
In this study, the monoclonal antibody-expressing CHO DG44 cell line was used under license from Cellca GmbH, Laupheim, Germany.
Chemical analyses
Chemical analyses were performed by Toxikon E…
Article
Leveraging Computational Models of Glycosylation for Biopharma QA
• Tandem α-1,3 galactose is a non-human glycosylation motif that is produced by murine cell lines, such as NS0 and Sp2/0 (9) and has also been observed in CHO cells (10). Presence of α-1,3 galactose r…