Search results for " CHO"
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Managing Residual Impurities During Downstream Processing
It incorporates choice of method; aspects of assay performance to be evaluated to demonstrate method validity, including critical reagent identification, retention, and planning; and provides general …
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Tools for Continuous Bioprocessing Development
Generally, production teams choose the platform that affords them the greatest control of the various process parameters. Without design rules for processes, this inevitably results in choosing platfo…
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Fed-batch supplements to boost cell culture performance
Cultures of Chinese hamster ovary (CHO) cells and the cholesterol-sensitive NS0 murine myeloma cell line were fed with HyClone Cell Boost™ nutrient supplements and HyClone LS1000 cholesterol supplemen…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
Cell line C is a CHO glutamine synthetase (GS) cell line. Frozen vials of the cell lines were thawed and grown in shake flasks containing Bristol-Myers Squibb’s proprietary basal media. The flasks wer…
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Evolution of the Monoclonal Antibody Purification Platform
Anion exchange (AEX) chromatography in a flow-through mode is the method of choice for removal of host-cell impurities. Hydrophobic-interaction chromatography (HIC) in bind and elute mode has been use…
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The Development and Application of a Monoclonal Antibody Purification Platform
HCP is detected using a high-sensitivity sandwich Enzyme-Linked ImmunoSorbent Assay (ELISA) kit specific for either CHO or NS0 proteins following the manufacturer's instructions (Cygnus Technologies, …
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A Closer Look at Affinity Ligands
Large-scale manufacturing of a therapeutic monoclonal antibody (mAb) starts with the expression of the antibody in mammalian cells, typically CHO [Chinese hamster ovary] cells, followed by a series of…
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Host cell protein quantitation using ELISA
This application note shows how using the sensitive HCPQuant™ CHO ELISA kit (Cytiva) and automating wash steps using Aquamax 4000 Microplater Washer (Molecular Devices) enables fast results without co…
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Optimizing your Cell Culture Process to Improve Productivity
It can be applicable to many different cell lines, including CHO or HEK, insect cells, and cell lines used in the cultivated meats industries. Furthermore, we do not need to know anything about the cu…
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Establishing a Robust Workflow to Identify High-Performing GOCHO™ Clones
Our cell line development platform uses the GOCHO™ host cell line to minimize the risk and timeline to develop high-performing clones suitable for manufacturing.
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