Search results for " CHO" in Articles / App Notes
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Development of Purification for Challenging Fc-Fusion Proteins
Development of Purification for Challenging Fc-Fusion Proteins
This study outlines methods for an alternative protein-polishing process for challenging proteins.
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Simple Fed-Batch Process Development for a Knockout Cell Line
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This workflow improved titers by 500% compared with Chinese hamster ovary cell culture in basal medium.
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News: Cytiva Adds ELISA Kit to End-to-End HCP Monitoring Capabilities
Cytiva is pleased to announce the new Amersham HCPQuant CHO kit, a generic for detecting host cell protein impurities in drug samples produced in CHO cell lines.
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Perfusion Culture Using TFF or ATF as Cell Retention Method
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Perfusion processes have many advantages such as being favorable for product quality and supporting efficient facility utilization by offering the possibility…
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Optimizing Late-Stage and Commercial Cell-Culture Processes
Whether these systems have the potential to disrupt the CHO hegemony for production of other proteins remains to be seen,” Racher says.
“These changes will eventually be at the level of the exp…
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Managing Residual Impurities During Downstream Processing
It incorporates choice of method; aspects of assay performance to be evaluated to demonstrate method validity, including critical reagent identification, retention, and planning; and provides general …
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Tools for Continuous Bioprocessing Development
Generally, production teams choose the platform that affords them the greatest control of the various process parameters. Without design rules for processes, this inevitably results in choosing platfo…
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Fed-batch supplements to boost cell culture performance
Cultures of Chinese hamster ovary (CHO) cells and the cholesterol-sensitive NS0 murine myeloma cell line were fed with HyClone Cell Boost™ nutrient supplements and HyClone LS1000 cholesterol supplemen…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
Cell line C is a CHO glutamine synthetase (GS) cell line. Frozen vials of the cell lines were thawed and grown in shake flasks containing Bristol-Myers Squibb’s proprietary basal media. The flasks wer…
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Evolution of the Monoclonal Antibody Purification Platform
Anion exchange (AEX) chromatography in a flow-through mode is the method of choice for removal of host-cell impurities. Hydrophobic-interaction chromatography (HIC) in bind and elute mode has been use…