Search results for "SEC "
Article
SEC in the Modern Downstream Purification Process
While SEC may have mostly been abandoned in monoclonal antibody (mAb) and other protein purifications, it is still commonly used in the purification of viruses and plasmids.
SEC, also known as gel…
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Analyzing Proteins Using SEC, MALS, and UHPLC
Today, SEC is predominantly used to measure aggregation, because the determination of protein molecular weight by calibrating the retention times against those of known molecular standards is not effe…
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Analytical SEC: How Does Back Pressure Relate to Resolution?
Let’s straighten out what really helps you achieve high-resolution analytical SEC data.
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Ensure SEC Column Reproducibility in Protein Drug Analysis
Size exclusion chromatography (SEC) is the go-to analytical method in the analysis of therapeutic protein drugs. This article describes the factors affecting the reproducibility of columns and how you…
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Selectivity in Size Exclusion Chromatography (SEC): It’s Much More than Average Pore Diameter (Å)
Describing the pore of a size exclusion chromatography resin with a single number such as average pore diameter is an oversimplification. The formation of pores is dependent on complex combinations of…
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Protein Characterization Using SEC: Three Webinar Takeaways
The possibility to separate molecules by size under native conditions is powerful in protein analysis. With more than thirty years in protein science, Åke Danielsson, Research Director at Cyti…
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Detecting Protein Aggregates and Evaluating their Immunogenicity
Users can add a light-scattering detector in line after SEC for irreversible aggregates, notes Some, and batch (unfractionated) light scattering methods can be applied to reversible aggregates. Analyt…
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Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
Samples subjected to the scale-down simulation were analyzed by SEC (after 1x and 3x freeze/thaw cycles). The 3x samples were also analyzed for subvisible particles by MFI.
RESULTS AND DISCU…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
We recently reported a practical SEC method for injecting samples onto the column in rapid succession and gating the detection window to monitor the elution of each sample individually.27 Generally, s…
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Virus-like Particles as Therapeutic Moieties of the Future
A SEC profile of a VLP is indicative of its structure, and any variation in it indicates possible variation in the structure of a VLP (23). NTA offers a non-invasive method to quickly analyze size dis…