Search results for "SEC "
Article
Modeling the Degradation of mAb Therapeutics
SEC analysis has shown that aggregates’ percentage was constant throughout the study, proving their minimal impact on the fragmentation process.
Sudden increase in fragmentation rate seen
In …
Article
Tackling Analytical Method Development for ADCs
Early development of cytotoxicity assays and chromatographic assays such as HIC, reduced reverse phase high-performance liquid chromatography (RPHPLC), and SEC is therefore important. She adds that ad…
Article
Protein Analysis with Size Exclusion Chromatography
This white paper presents fundamentals about analytical SEC and describes how our new-generation agarose-based SEC columns are used in analysis of biomolecules.
Download white paper
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
…omes followed by gentle size exclusion chromatography (SEC) with our next-generation Cytiva™ superSEC resin. We found that:
- Cytiva™ superSEC resin enriched exosomes from impurities compared to l…
Article
Impurity Testing of Biologic Drug Products
Experts share insights on the various methods used for purity and impurity analysis of therapeutic proteins.
By Adeline Siew, PhD
CA-SSIS/shutterstock.comIm…
Article
Development of Purification for Challenging Fc-Fusion Proteins
…mance liquid chromatography (UHPLC) (Waters) with Empower software that used an Acquity UPLC BEH200 SEC column. The protein was monitored by UV280 nm.
LMW fragment quantification was per…
Article
Column Tips for Successful SEC-MALS
Do you identify molecules using MALS, maybe in combination with size exclusion chromatography (SEC)? We have gathered Column Tips for Successful SEC-MALS in a blog post. Tap into these column …
Article
Protein Purification Troubleshooting Guide
Practical advice to significantly impact your chromatography workflow
Put this poster on your lab wall to quickly identify and resolve protein purification problems when and where they happen. I…
Article
Selection Guide Size Exclusion Chromatography Columns and Media
Size exclusion chromatography (SEC), also called Gel filtration (GF), separates molecules on the basis of differences in size as they pass through a SEC medium packed in a column. A variety of media…
Article
USP Publishes Monoclonal Antibody Guidelines
Mar 02, 2015
By Dale Schmidt, M.S., Anita Y. Szajek, PhD, Fouad Atouf, PhD, Tina S. Morris, PhD
BioPharm International
Volume 3, Issue 28
As FDA gears up towards approving biosimilar dru…