Search results for "Protein A" in Articles / App Notes
Article
As the therapeutic pipeline diversifies, chromatography toolboxes are expanding.
For traditional mAbs, protein A chromatography is used to capture antibodies. Depending on the structure of the target antibody and the impurity profile, other affinities, like protein L or variants o…
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Choose your Optimal Wavelength for Chromatography
U9-T monitor allows differentiation between peaks containing protein and those containing DNA/RNA, nucleic acids or nucleotides. Most proteins absorb strongly at 280 nm due to the presence of tryptoph…
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Chromatography for the Diversified Antibody Pipeline
We can see the protein and understand the necessity for having other tools targeting regions on the antibodies such as Kappa ligand or the Lambda ligand. These were developed for fragments and not alk…
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Enhancing Resins Addresses Purification Concerns
Protein A affinity chromatography provided one simple capture step that made screening of clones faster and purification process development simpler,” he states.
Deorkar also explains that p…
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Cytiva Advances Fiber Chromatography Technology with New Launch
The product complements HiTrap Fibro PrismA, the company’s new fiber-bases Protein A platform launched for research applications in 2020.
The Fibro technology features an open porous adsorbent mat…
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Updating Viral Clearance for New Biologic Modalities
Guidance is needed
Compounding all these challenges is the fact that published regulatory guidances have been based on traditional biologics, such as mAbs and related Protein A binders, says Berri…
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Western Blot Detection: Is Film or a Digital CCD Imager Better?
Western blotting, known also as immunoblotting or protein blotting, is a well-established and widely accepted form of protein detection and analysis. It is based on building an antibody:protein comple…
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Using Multiple Techniques in Biosimilar Analysis
Application of multiple techniques at different conditions presents a more complete picture of a dynamic situation.
By Rebecca Strawn
Sergey/stock.adobe.com
Almost all drug product…
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How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
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In protein purification, dynamic binding capacity (DBC) of a chromatography column describes the maximum amount of target protein that you can load onto your co…
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Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The host cell protein amount per dose and strain in the scaled-up production was also shown to be below acceptance level of WHO. The outcome from the scaled-up production indicates that it is possible…