Search results for " mammalian"
Article
Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
… neutralization and size-exclusion chromatography of recombinant antibodies expressed transiently in mammalian human embryonic kidney 293T-cells at the 1-L scale. The system consists of two bench-top…
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Fifteen Years of Progress: Biopharmaceutical Industry Survey Results
Trends in adoption of diverse mammalian expression systems have also not materialized, as Chinese hamster ovary (CHO) cell lines have become the dominant expressions systems. In fact, nearly 80% of re…
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The Outlook for CMO Outsourcing in 2019
Survey data and trends
Developers generally outsource a higher percentage of projects/products involving mammalian vs. microbial expression systems. The historical picture of respondents to …
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
Major mammalian expression systems for the production of biotherapeutics include NS0 cells, baby hamster kidney (BHK) cells, hybridoma cells, PER.C6 cells, human embryonic kidney (HEK) cells, and Chin…
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Antibody Production in Microbial Hosts
However, use of mammalian expression system also has few disadvantages, which has propelled manufacturers to search for alternative host systems. One of the most important challenges is predicting the…
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New Therapies Present Scaling Challenges
In addition, mammalian cell culture technology has advanced such that producing multi-kilogram quantities of therapeutic mAbs in one batch is now commonplace (1). With a cell therapy, however, establi…
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Development of Purification for Challenging Fc-Fusion Proteins
Introduction
Improvements have been made in mammalian cell culture to achieve high productivity processes primarily to meet the increasing demand of biological therapies. In response, tit…
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Implications of Cell Culture Conditions on Protein Glycosylation
In mammalian cell culture processes, the use of different species can potentially produce significant differences in the types of glycosylation that can occur. These differences in glycosylation can h…
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Advanced Technologies Facilitate Scale-up and Technology Transfer
Fujifilm develops and manufactures biologics using both microbial and mammalian production systems, and although single-use technology is a relatively new introduction, it has had an impact on mammali…
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Being Thorough When Transferring Technology
Lonza: When scaling up mammalian processes from lab scale to commercial, the decline phase of the production culture can be faster at larger scale, leading to a premature reduction in product accumula…