Search results for " capture" in Articles / App Notes
Article
Host-Cell Protein Measurement and Control
In Figure 1, capture antibodies enrich the HCPs from sample and immobilize them to a 96 (or greater)-well plate. Then, detection antibodies, conjugated directly with an enzyme or through a biotin-avid…
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Detecting Protein Aggregates and Evaluating their Immunogenicity
LO alone is not sufficient to detect aggregates, as it is not sensitive enough to always capture translucent particles. Aggregation can cause protein particles to become transparent, and relying solel…
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Comparing Protein A Resins for Monoclonal Antibody Purification
Protein A is the affinity chromatography ligand of choice for the first antibody capture step, because its high selectivity gives excellent purity (typically > 99%) and high yields. Furthermore, Prote…
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Evaluating the Use of Continuous Chromatography
…an overload the first column [because] any product/target molecule that is breaking through is being captured by the second column in the loading zone. When used with a perfusion or fed-batch bioreac…
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A Closer Look at Affinity Ligands
…ioCina): Affinity ligands can produce a very high purification factor in a single unit operation and capture product from a dilute process stream. This makes affinity purification very attractive in …
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Top Tips for Successful Development of Antibody Chromatography Processes
But working with protein L, that only binds kappa subclass one, three and four, for instance, MabSelect™ VL, you could get a really high purity sample during the capture step. Since the lambda heterod…
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Chromatography innovations for the diverse antibody pipeline
In this video Josefin Bolik, global product manager — antibody capture at Cytiva, presents how to select resins for your antibody variant purification project.
WATCH THE VIDEO >>
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Insider Recommendations for Getting Started with Mechanistic Modeling of Chromatography
That completely captures all of the fluid dynamics and, of course, the internal geometry of the column and system.
Once we've defined these fluid mechanical effects, we can then start to provid…
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Track-and-Trace Progress Benefits Supply-Chain Security Efforts
…cations, and share data electronically using electronic product code information services (EPCIS) to capture events from manufacturing to serialization [and] capping to shipping.EPCIS can capture tho…
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Setting Up Bioprocessing Systems for Digital Transformation
Thus, staying compliant means ensuring that the connectors between systems capture all that is needed and that they do not manipulate data. “Many organizations are looking to data lakes to help meet A…