Search results for " electrophoresis" in Articles / App Notes
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
CIEF usually is performed on commercial capillary electrophoresis instruments, which have a 20–60 cm long capillary and on-column ultraviolet (UV) absorbance detector. Imaged CIEF, or iCIEF, which use…
Article
N-Glycan Analysis of Biotherapeutic Proteins
Capillary electrophoresis (CE)-electrospray ionization (ESI)-mass spectrometry (MS) (CE-ESI-MS) may also be used to profile intact glycoform masses (28).
Figure 2: Analytical approach…
Article
Host-Cell Protein Measurement and Control
Supplementary to HCP ELISA, traditional HCP separation and visualization methods, such as 1D and 2D sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), remain useful tools for HCP ch…
Article
Virus-like Particles as Therapeutic Moieties of the Future
Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) can be used to analyze the molecular weight and protein purity of VLPs. The identity of individual capsid proteins can be confirme…
Article
Impurity Testing of Biologic Drug Products
…e and qualitative analyses of N- and O-glycosylation
• Liquid chromatography or electrophoresis methodologies to evaluate product heterogeneity (charge variants, size variants, hydropho…
Article
Getting a Handle on Biopharma’s Most Critical Quality Attributes and Quality Control
Conventionally, methods that include peptide mapping, N-glycan analysis, capillary electrophoresis (CE), ion exchange chromatography, reversed-phase ultra-high-performance liquid chromatography (RP–UH…
Article
Nurturing Knowledge from Disparate Data Streams
Difficulties in MS and NMR
Separation techniques such as gas chromatography (GC), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and gel electrophoresis when couple…
Article
Development of Purification for Challenging Fc-Fusion Proteins
The samples in the collection plate were then analyzed by an ultraviolet (UV)-vis spectrophotometer and electrophoresis on a chip (Perkin Elmer) in a 96-well format. All experiments were performed at …
Article
USP Publishes Monoclonal Antibody Guidelines
This chapter includes purity assessments by chromatographic separation of size variants and capillary sodium dodecyl sulfate (CE–SDS) electrophoresis. In addition, it includes procedures for analysis …
Article
Detecting Protein Aggregates and Evaluating their Immunogenicity
This technique helps link liquid chromatography or capillary electrophoresis electrospray (CESI) easily and allows orthogonal techniques to work together for better information content. With peptide m…