Search results for " concentration"
Poster
Guide to modern BioProcess™ chromatography resins
Proteins are different. Based on what protein you would like to purify and the conditions used, chromatography resins are selected to optimize purity and yield. Many bioprocess applications include …
Article
Perfusion in the 21st Century
If specific productivities are kept high, higher cell concentrations (generally, greater than 100 x 106vc/mL) can be attained resulting in greater volumetric productivity (more than 1.5 g/L day). Incr…
Article
Make buffer preparation more time and cost efficient with inline conditioning
Simplify Buffer Preparation
The volume and number of buffers for a typical downstream process can be considerable.
Preparation of these buffers is a challenge that in-line conditioning can o…
Article
An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
Chemically defined raw materials have all components identified; components are in the chemical form, and the structure and concentration of all components is known and of high purity with only minima…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
…4), and the mutation rates measured by 6-thioguanine (6-TG) assay positively correlated with the MTX concentrations used to select the recombinant cell lines (3). Because multiple rounds of amplifica…
Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
This method, widely named trypsinization, is based on the addition of an active concentration of the enzyme to the cell culture and subsequent digestion of cell-membrane proteins that establish the in…
Article
Robust Optimization, Simulation, and Effective Design Space
Figure 1: Robust optimization for a target concentration.
Step Seven: Optimize the process and define the set points
When determining the recipe for a formulation or process (set points) there…
Article
Biopharma Advances Demand Specialized Expertise
The main roadblock for continuous processing is downstream processing of proteins, which currently demands discrete and distinct unit operations for removal of impurities, removal of contaminants, con…
Article
Advanced Technologies Facilitate Scale-up and Technology Transfer
Single-use and modular technologies plus continuous manufacturing are increasingly important to biopharma scale-up and tech transfer.
Jun 01, 2015
By Cynthia A. Challener
BioPharm Inte…
Article
Modular Manufacturing Platforms for Biologics
May 01, 2015
By Randi Hernandez
BioPharm International
Volume 28, Issue 5
It should come as no surprise that the good design principles governing the execution of pharmaceutical manu…