Search results for " downstream"

Article Handbook: Size Exclusion Chromatography Principles and Methods
This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection crite…

Poster Optimization of concentration and diafiltration of a bovine γ-globulin solution
This application note describes the development of a cross flow filtration process for concentration of a bovine γ-globulin solution.

Article Detecting Protein Aggregates and Evaluating their Immunogenicity
Mar 01, 2015 By Randi Hernandez BioPharm International Volume 3, Issue 28 Aggregation in biopharmaceuticals remains a major concern and threatens the stability of a product. Protein …

Poster Accurate comparability assessment of a biosimilar interferon in process development
This application note describes how to achieve accurate comparability assessments using Biacore™ T200 and Amersham™ WB system in the development of a biosimilar. This is exemplified in this study…

Article Using Quality by Design to Develop Robust Chromatographic Methods
Quality-by-design principles can be used to understand chromatographic measurement system variability. Sep 2, 2014 By: Melissa Hanna-Brown, Kimber Barnett, Brent Harrington, Tim Graul, Jim…

Article Concentration and Diafiltration of Cell-Derived, Live Influenza Virus Using 750 C Hollow Fiber Filter Cartridge
Cross flow filtration (CFF) is a technique extensively used in vaccine production and particularly in influenza vaccine manufacturing. In contrast to normal flow (dead-end) filtration (NFF), the fee…

Article Single-Use and Continuous Processing Technologies Change Facility Design
Viewing Changes in Pharmaceutical Facility Design Single-use systems, continuous processing and isolator technology use a smaller footprint. Experts predict how the facilities of the future may …

Article Biopharma Outsourcing Activities Update
This increased outsourcing is also the case for areas such as cell line development and downstream process development. Moreover, expectations surrounding future levels of outsourcing activity rema…

Article Implementing QbD in Sterile Manufacturing
To gain perspective on the implementation of quality by design (QbD) in sterile manufacturing, BioPharm International spoke with Wolfgang Weikmann, vice-president quality assurance at Vetter Pharma-Fe…

Article Evolution of the Monoclonal Antibody Purification Platform
The authors discuss the evolution of the purification platform for manufacturing of mAb therapeutics. Monoclonal antibodies (mAb) increasingly form the majority share of the product pipeline…

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