Search results for " titer" in Articles / App Notes
Article
Putting Viral Clearance Capabilities to the Test
Achieving a satisfactory clearance level can be limited by the virus titer, by cytotoxicity, or by viral interference,” she explains. “These limitations can be addressed by careful study design to opt…
Article
Cleaning Protein A Resins with 1 M NaOH
With increasing mAb titers, the impurity level of the cell culture feed increases. If not removed, these impurities can build up on the protein A resin and start to leach into the process material i…
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Single-Use Chromatography: Adapting to Manufacturing Scale
…acturing scale?
This article discusses strategies for meeting the ever-increasing upstream titers and volumes to prevent downstream purification from becoming a bottleneck.
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Article
Modeling and Miniaturization: Key Topics at Successful HTPD Conference
Top producing clones were selected by titer and growth and could potentially be ready to be scaled up to bioreactor.
Winning Poster
The second day of the conference concluded with the post…
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Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
Human embryonic kidney 293 (HEK293) cells are widely used in production of recombinant viruses and proteins because of their stable growth, high transfection level, and ability to produce high viral t…
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Increasing upstream titers can make the Protein A chromatography process step the rate-limiting step in downstream purification. Protein A chromatography columns are frequently too large, limiting the…
Article
Process Chromatography: Continuous Optimization
“An increase in binding capacity will be beneficial for process economy reasons when purifying steadily increasing upstream titers,” he states. Current protein A resins are also exposed to feeds with …
Article
Unifying Continuous Biomanufacturing Operations
Advancements in sensors informing operators on product quality, glycosylation, viral titer, viral antigen amount, and osmolality, specifically, would be of great use to the industry, noted Genzel, and…
Article
Being Thorough When Transferring Technology
Other examples are unexpected low titer compared to process development runs or unexpected filter clogging in depth-filtration steps due to different quality of filtration starting material. An exampl…
Article
Speed and Flexibility Are Dual Goals for Biopharma Operations
…ions—such as advances in bioreactor technology, media, buffers, and cell lines that produce higher titers in smaller systems—reflected an evolution in technology. Other product introductions included…