Search results for " Protein A"
Poster
Columns up to 1600 mm in diameter packed with protein A chromatography medium using axial mechanical compression
Axial compression allows for optimally packed beds also in the large-scale chromatography column range. Different methods have been developed to pack a broad range of chromatography media (resins) i…
Resource
Halle Conference on Recombinant Proteins, Halle, Germany
Feb 19-20, Halle, Germany
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
The cost of the virus-retaining filter, which can approach that of the protein A resin, can be reduced by using prefilters or optimizing protein concentration, temperature, buffer composition, and sol…
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Impurity Testing of Biologic Drug Products
“Product-related impurities can be categorized as product variants, and basically correspond to any undesired modification of the protein amino-acid sequence or post-translational modifications,” she …
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Impact of Media Components on CQAs of Monoclonal Antibodies
Cysteine, along with temperature shift, is believed to reduce protein aggregates and increase their stability in culture harvests. The oxidized form of cysteine has been shown to reduce the formation …
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Modeling the Degradation of mAb Therapeutics
Mathematical modeling of protein aggregation correlated along with kinetic data can help developers gain both qualitative and quantitative insights into the mechanism behind aggregation. Armed with th…
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Generating a Fully Processed Antibody
The authors outline cell-line development and process scale-up for an antibody program in which the antibody requires additional processing by a site-specific enzyme for correct functionality.
B…
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A Closer Look at Affinity Ligands
The most common example in the realm of bioprocessing is Protein A, which has an extremely high affinity for some immunoglobulin molecules. Other examples include antibody/antigen, enzyme/substrate, a…
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Elucidating Biosimilars Characterization
This is especially so for recombinant human proteins, or ‘humanized’ antibodies (antibodies that have a significant part of their protein amino-acid sequence from human origin), as they are highly imm…
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Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
The freezing rate in a single freeze-thaw cycle had negligible impact on protein aggregation when fast-thawing conditions were used to thaw. Slow thawing led to higher protein aggregation and subvisib…