Search results for " Transfection" in Articles / App Notes
Article
Recombinant Adeno-Associated Virus Type 5 Production Process
A scalable recombinant adeno-associated virus (rAAV) type 5 production process was developed using transfected HEK293 suspension cells.
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Article
Top Challenges to Overcome When Developing AAV Processes: Part 1 of 2
…ich development platform to use and scalability
3:09 – Parameters evaluated for AAV plasmid transfection
4:27 – Three main challenges for downstream AAV process development, including c…
Article
Genetic Vaccine Platforms Demonstrate Their Potential
“Methods exist, but these generally are research-based techniques (such as sequencing, infectivity, transfection assays), and they need to be fully developed to be robust, validatable, GMP-compliant, …
Article
Strategizing for Rapid Changeovers in Biologics Manufacturing
Olivier Le Moal/Stock.Adobe.com
By Cynthia A. Challener
Facility and equipment design are important, but the team and its experience matter most.
Through its Operation Warp Speed pro…
Article
Design Considerations for a Commercial Cell and Gene Therapy Facility
Process-specific equipment are typically cell isolation, selection, and transfection systems,” Ramaswamy clarifies.
Single-use systems are technologies that are widely used in the bioprocessing …
Article
Scalable Viral Vector Manufacturing
…s and Adeno-Associated Virus, the virus can be produced upon introduction into the host cell line by transfection of plasmids encoding both the viral genes and the gene of interest. Thus, the plasmid…
Article
Optimizing Late-Stage and Commercial Cell-Culture Processes
Optimizing Late-Stage and Commercial Cell-Culture Processes
Late-stage and commercial biomanufacturing pose a challenge to cell-culture processing.
By Feliza Mirasol
…
Article
Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
Human embryonic kidney 293 (HEK293) cells are widely used in production of recombinant viruses and proteins because of their stable growth, high transfection level, and ability to produce high viral t…
Article
Single-use Bioreactors Have Reached the Big Time
The decision to use disposable bioreactors is now driven by commercial rather than technological considerations.
By Cynthia A. Challener
Single-use bioreactors available from various vendors…
Article
An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
Testing product and process intermediates alone is helpful, but does not provide a complete solution to viral safety. This article proposes integrated solutions for systemic and proactive viral risk m…