Search results for " medium"
Poster
Effects of feeding strategy on CHO cell performance in fed-batch cultures
ActiPro cell culture production medium is intended to be used in combination with Cell Boost 7a and 7b supplements to enhance recombinant protein production in fed-batch or perfusion processes. This…
Poster
Validation of Cytodex™ Gamma microcarrier-based virus production in single-use bioreactor systems
Here, we describe different approaches for the cultivation of Vero cells using Cytodex and Cytodex Gamma microcarriers in serum-free cultivation medium using spinners, WAVE Bioreactor™ 20/50, ReadyToP…
Article
Modeling Bioreactor Performance
In general, process-specific data must also be generated to perform these modeling studies because oxygen transfer and mixing times differ depending on the medium used and the bioreactor fill volume a…
Article
Mixing dynamics in a large volume single use mixer
Upstream applications such as medium preparation and harvest require fast and efficient mixing whereas downstream mixing operations used in purification and separation processes require sensitive mi…
Article
Selection Guide Size Exclusion Chromatography Columns and Media
Size exclusion chromatography (SEC), also called Gel filtration (GF), separates molecules on the basis of differences in size as they pass through a SEC medium packed in a column. A variety of media…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
… improving therapeutic protein production systems with a focus on host-cell engineering,cell-culture medium, and process development.
While the aforementioned efforts successfully introduced clini…
Poster
Modes of controlling charge variant distribution in Mab production
In this study, we investigated the effects of cell culture process parameters and medium components on charge variant distribution of a human IgG1 MAb. Furthermore, the MAb charge variant clearance in…
Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
…emporal resolution due to the formation of reactive oxygen species (ROS) that diffuse in the culture medium (13). Efforts in this direction have led to the development of a plasmonic substrate that p…
Article
Antibody Production in Microbial Hosts
The authors review the status of expression of antibodies in microbial hosts and present the recent advances in the production of aglycosylated antibodies in bacteria.
By Anurag S. Rathore, Jyot…
Article
Using Xcellerex™ mixing system as a slurry tank when packing chromatography columns
The capability of the mixing system to keep the chromatography medium (resin) in suspension and the possibility to integrate the system in column packing were studied. Further, an evaluation of the ef…