Search results for " size-exclusion"
Article
Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
Soluble aggregates and subvisible particle counts were measured by size-exclusion chromatography and micro-flow imaging, respectively. Thermal analysis of protein samples was performed by modulated di…
Article
Virus-like Particles as Therapeutic Moieties of the Future
…h multiple angle light scattering (AF4–MALS), dynamic light scattering (DLS), disc centrifugation, size-exclusion chromatography (SEC), analytical ultracentrifugation (AUC) (15), and nanoparticle tra…
Article
Process Chromatography: Continuous Optimization
Metal chelate, size-exclusion, and hydroxyapatite resins are also used on a limited basis, according to Andrew Bulpin, head of process solutions strategic marketing and innovation with MilliporeSigma.…
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
If used correctly, these new analytical methods can reduce analysis and product development time.
ABSTRACT
Monoclonal antibodies represent a significant portion of sales in the biopharma…
Article
A Closer Look at Affinity Ligands
The same drawbacks apply to purification processes that do not rely on ligands, such as the size separation performed by size-exclusion chromatography or filtration.
In addition, affinity separati…
Article
Enhancing Resins Addresses Purification Concerns
However, precipitation or size-exclusion chromatography (SEC) are not cost-effective when scaled up,” Deorkar says.
Affinity chromatography resins such as oligo(dT) can be…
Article
Moving PAT from Concept to Reality
The learning curve for process analytical technology has slowed widespread adoption.
By Cynthia A. Challener
alexlmx a- Stock.Adobe.com
Process analytical technology (P…
Article
Getting a Handle on Biopharma’s Most Critical Quality Attributes and Quality Control
Among the most widely used methods are peptide mapping, which can identify and quantify critical PTMs; size-exclusion chromatography, which can help determine the degree of aggregation, and N-glycan a…
Article
Modeling the Degradation of mAb Therapeutics
The samples were analyzed for aggregation content using size-exclusion high performance liquid chromatography (SE–HPLC) and types of oligomers using dynamic light scattering (DLS) (7) (Figure 1A).
…
Article
Using Multiple Techniques in Biosimilar Analysis
Similarly, for reporting on quaternary structure, analytical ultracentrifugation (AUC) and size-exclusion chromatography with multi-angle static light scattering (SEC-MALS) are commonly used orthogona…