Search results for " recombinant"
Article
QbD Improves Cell-Culture Process Control
QbD Improves Cell-Culture Process Control
A quality-by-design approach that implements PAT offers advantages in upstream cell-culture processing.
By Feliza Mirasol
Mar…
Article
Roadmap Leads to Innovative Biomanufacturing Strategies
The initial emphasis of the Biomanufacturing Technology Roadmap has been on the manufacture of recombinant proteins and, even more specifically, on monoclonal antibodies (mAbs).
With successive …
Article
Reducing Cross-Contamination Risks in Process Chromatography
Preferred target molecules
A variety of biologics benefit from the use of disposable technology in their production, from monoclonal antibodies (mAbs) and recombinant medicines to vaccines and…
Article
ADC Development Robust Despite Lackluster Performance
With nigrin, the company has developed two payloads: a nigrin b-A chain molecule and a recombinant version of the nigrin b-A chain. Nigrin b is a plant toxin derived from the bark of an elder plant (S…
Article
Development of Purification for Challenging Fc-Fusion Proteins
Materials and Methods
Purification techniques
Feed stock: Fc-fusion proteins with an isoelectric point (pI) ranging from four to seven were expressed in recombinant Chinese h…
Article
Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
Human embryonic kidney 293 (HEK293) cells are widely used in production of recombinant viruses and proteins because of their stable growth, high transfection level, and ability to produce high viral t…
Article
Process Chromatography: Continuous Optimization
… purification process making it simpler and more economical for a variety of molecules from mAbs, to recombinant proteins, to viruses.
Hydrophobic interaction resins separate molecules based on d…
Article
Managing Residual Impurities During Downstream Processing
Some specifics on residual HCPs
The presence of HCPs in a biologic material is inevitable given that the main route of production for biopharmaceuticals is through recombinant technology via a host…
Poster
Development of DoE based fed-batch strategies for high-producing CHO cell cultures
To mimic more closely the dynamics of a fed-batch culture, we developed a dynamic feeding strategy responsive to the actual nutrient needs of a mAb-producing recombinant Chinese hamster ovary (CHO) ce…
Article
Fusion Proteins Pose Manufacturability Challenges
Making proteins ‘druggable’
Many proteins have short half-lives in human serum, making them unsuitable as recombinant protein drugs. When constructed as fusion proteins, however, they may become ‘…