Search results for " recombinant"
Article
Tools for Continuous Bioprocessing Development
Cells were genetically modified to produce different recombinant proteins in response to different chemical inducers and it was shown that by testing the synthetic circuits in turbidostatic steady sta…
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Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
Data were available for 15 measurements over 16 time points for 20 cultures (19 Chinese hamster ovary cell lines) making the same recombinant monoclonal antibody (mAb) giving a 240-dimensional data se…
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GMP Challenges for Advanced Therapy Medicinal Products
“Manipulation of cells and use of recombinant nucleic acids may bear unknown risks, which may not be solvable through standardization or quality control,” Salmikangas added.
A major problem area wi…
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Implications of Cell Culture Conditions on Protein Glycosylation
The choice of host-cell line for recombinant protein production depends first on the protein’s molecular properties. Certain bacteria can be used for production of the simplest proteins, those that ar…
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Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
Advanced antibody engineering has resulted in the ability to manufacture new recombinant bispecific antibodies suitable for therapeutic use. FDA has approved a bispecific antibody—Amgen’s Blincyto (bl…
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Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…
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Re-use of Protein A Resin: Fouling and Economics
Mar 01, 2015
By Anurag S. Rathore, Mili Pathak, Guijun Ma, Daniel G. Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography ha…
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Ligand-Binding Assays and the Determination of Biosimilarity
Therefore, the company immobilized all six recombinant Fc receptors on nickel-coated biosensor tips for use in BLI analyses. “With this approach to binding kinetic analysis, the KD values for mAbs, bo…
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Viral Clearance Challenges in Bioprocessing
…ice/porcine parvovirus [MVM/PPV]) is applied for the viral clearance validation of cell line-derived recombinant products. The achieved single reduction factors are summed up to an overall reduction …
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Setting Standards for Biotech Therapeutics in India
Approval of the first recombinant DNA product, human insulin, occurred only in 1982. The experience is even more limited in India. The government, through agencies such as the Department of Biotechnol…