Search results for " mammalian"
Article
Ensuring the Biological Integrity of Raw Materials
Mycoplasmas are the smallest of free-living organisms, and are frequent contaminants of mammalian cell cultures. They can alter the metabolism and properties of cells and change product yield, cause f…
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Generating a Fully Processed Antibody
Formylglycine-generating enzyme (FGE) is an enzyme expressed in virtually all mammalian cells as well as most other eukaryotic and prokaryotic cell types (1). In the endoplasmic reticulum during prote…
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Analysis of Glycosylation in Biosimilars
The mammalian cells that are typically used for the culture can produce up to four ‘arms,’, or tetra-antennary structures. These structures can be seen at the high mass (right) side of the upper mass …
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Managing Biomanufacturing Capacity Expectations
According to numbers from the 2016 BioPlan report (1), approximately 59% of respondents used at least some outsourced capacity for mammalian cell culture, 55% used outsourcing for microbial fermentati…
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Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
Will the facility be manufacturing monoclonal antibodies, recombinant proteins, vaccines, antibody-drug conjugates, or fragment antibodies? Also, will the products be mammalian cell-derived or microbi…
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A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Evaluation of integrity of the transgene mRNA expressed in CHO cells
RNA splicing is a natural process in mammalian cells that removes introns and joins exons in a primary transcript to create matu…
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Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
A typical bioprocess for a mammalian cell line may have 170 process factors. Using this approach, a 170-dimensional space would require 3.3 x 10119 cultures for full characterization, which is not fea…
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Best Practices in Qualification of Single-Use Systems
Vinci and S Parekh, Handbook of Industrial Cell Culture: Mammalian, Microbial and Plant Cells (Humana Press, 2003).
13. P. Culter, Protein Purification Protocols, Second Edition (Humana Press, 200…
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Biopharma Advances Demand Specialized Expertise
…hat are the roadblocks to implementation?
Lorimer (Patheon Biologics): Continuous production from mammalian cell cultures has been operated in perfusion bioreactors for many years. The main roadbl…
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Host-Cell Protein Measurement and Control
Although not every host gene will be transcribed and translated to protein, the complexity of host genome and the post-translational modification present in mammalian cells make it almost impossible t…