Search results for " columns"
Article
The Development and Application of a Monoclonal Antibody Purification Platform
The percent monomer is determined by size exclusion high performance liquid chromatography, using two G3000 SWXL columns (Tosoh, Montgomeryville, PA) in series with detection of the antibody at 214 nm…
Article
Extractables in Single-Use Systems Used in ADC Manufacturing, Part 2
The low levels of extractables found in this study support the use of ReadyToProcess™ columns, ÄKTA readyflux™ flow kits, and ÄKTA ready™ flow kits in ADC processes.
Read the application …
Article
Discovering the unknown: How does in silico process development change PD scientists’ work?
In silico process development for chromatography is a paradigm-shifting approach that redefines the routine work of process development scientists. But what does it actually mean to perfor…
Article
Report from the 6th International HTPD Conference
Two challenges highlighted were the current practice of using microscale columns since the flow rate is limiting for viral clearance in short columns, and that the precision in the models is not there…
Article
Digitalization: The Route to Biopharma 4.0
Industry 4.0, also known as the Fourth Industrial Revolution (4IR), will result in the digital transformation of the manufacturing sector. It is propelled by transformative trends such as the prolif…
Article
Updating Viral Clearance for New Biologic Modalities
Viral clearance processes and guidance must evolve along with newer biotherapeutic modalities.
By Feliza Mirasol
Ensuring viral clearance (i.e., removal) in downstream purification has becom…
Article
Strategizing for Rapid Changeovers in Biologics Manufacturing
Stoll adds that some product-dedicated equipment must also be installed, in particular chromatography columns with packed resin for drug substance purification. “All these activities may potentially b…
Poster
Comparison of Three Different Screening Techniques for Predicting Step Elution Conditions for a Cation Exchanger
Screening of chromatographic conditions can be done in several ways, such as 96-well plates, mini-columns, and tips. It is important that the screening can give an indication of the optimal conditio…
Article
A Look at the Affinity Chromatography Landscape
Affinity chromatography for DNA-based biologics
Methacrylate monolithic columns have been an enabling tool for developing fast and efficient separation processes for DNA-based biologics with low …
Poster
A Next Generation Protein A Chromatography Resin for Improved Productivity and Bioburden Control
mAb production using a Protein A capture step has followed a highly successful synergistic path the last 30 years. There are, however, remaining challenges.
• The increased upstream titers in mA…