Search results for " bovine" in Articles / App Notes
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Efficient concentration of a low-titer bovine IgG with high recovery in low volume
The described process enabled concentration of a bovine IgG antibody from 50.0 to 0.165 L in less than 5 h.
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Ensuring Viral Safety of Viral Vaccines and Vectors
Unscreened human or animal-derived products, such as bovine serum, are now known as potential sources of bovine virus contamination (1).
Since then, better safety measures and the use of establ…
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An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
Examples include: bovine serum albumin (BSA), human serum albumin (HSA), porcine trypsin, purified enzymes, wool-derived cholesterol, heparin, gelatin, casein, and collagen.
Secondary …
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Ensuring the Biological Integrity of Raw Materials
For example, bovine serum can be contaminated with reovirus, epizootic hemorrhagic disease virus, Cache valley virus, or Vesivirus 2117. Porcine circovirus is sometimes found in porcine trypsin. Minut…
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Recent Advances in the Use of Exoglycosidases to Improve Structural Profiling of N-glycans from Biologic Drugs
…cetylglucosamine (GlcNAc) of a released N-glycan inhibits enzymatic removal of core α 1,6 fucose by bovine kidney fucosidase (BKF), the enzyme historically used to confirm the presence of this import…
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Safety Drives Innovation in Animal-Component-Free Cell-Culture Media Technology
Replacing complex bovine serum supplements with purified bovine proteins such as albumin, insulin, transferrin, and fetuin was seen as a major advantage in the early days of serum-free media technolog…
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Implications of Cell Culture Conditions on Protein Glycosylation
An example of this can be seen in the N-glycans released from bovine fetuin, as shown in Figure 2. This method gave consistent data across three preparations, a series of clustered peaks representing …
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Application of Quality by Design to Viral Safety
For instance, massively parallel sequencing has been used to evaluate bovine serum, and this testing helped distinguish bovine adeno-associated virus (BAAV-2) as a new contaminant (5). Once a panel of…
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Report from the 12th Plasma Product Biotechnology Forum
…umanized, highly potent polyclonal antibodies without the need for human serum, as they are based on bovine plasma from transchromosomic cows. Despite the unusual raw material, there is a well-establ…
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HyClone Characterized FBS – Origin Equivalence Study
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Fetal bovine serum (FBS) is still used in many cell culture applications, including viral vector production. FBS from a single origin may be limited, so qualifying FBS from …