Search results for "Recovery" in Articles / App Notes
Article
A Q&A With Günter Jagschies: Recovery of Biological Products Conference Series
Could you share your viewpoint on the importance of this meeting?
The Recovery of Biological Products is probably the meeting most relevant to scientists, engineers, and managers in the bioprocess…
Article
Single-Use Workflow for Recovery of a Domain Antibody from E. coli Culture Feed in an Automated Manner
This application note demonstrates the performance of the automated ÄKTA™ readyflux single-use filtration system in microfiltration applications. Here, a domain antibody (dAb) expressed in E. col…
Article
Troubleshooting Protein Recovery Issues
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Explore common reasons for protein recovery issues, and how to fix them to ensure you obtain the amount of pure protein that you expect.
Article
Dear lentivirus: We have not forgotten about you and how to improve your polishing recovery
Lentiviruses are commonly used for both chimeric antigen receptor (CAR) T cell therapy and ex vivo gene therapy.
Vector purification is very challenging due to low stability of these envel…
Article
Efficient concentration of a low-titer bovine IgG with high recovery in low volume
This application note describes the design of circuits with low working volume, allowing for high concentration of dilute feed streams. The use of ReadyCircuit™ single-use assemblies allowed for as…
Article
Evaluating Surface Cleanliness Using a Risk-Based Approach
The process typically involves performing 100% spray coverage testing, rinse recovery testing, specific or non-specific analytical method qualification, surface sampling and recovery testing of the sa…
Article
Development of Purification for Challenging Fc-Fusion Proteins
… protein polishing step successfully implemented at large scale, which demonstrates acceptable step recovery and efficient clearance of impurities for three different Fc-fusion molecules.
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Article
Establishing Acceptance Criteria for Analytical Methods
Traditional measures of analytical goodness include the following:
• % coefficient of variation (CV) = (repeatability/mean)*100
• % recovery = (measured concentration/standard concentration)*100
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Article
PDA's Technical Report for Biotech Cleaning Validation
Sampling recovery studies for biotech cleaning validation are not different in principle from sampling recovery studies in nonbiotech cleaning validation. However, residues used for spiking surfaces f…
Article
Gene Therapies Push Viral Vector Production
…and for AAV specifically, improve ratio of full versus empty capsids; as well as improve downstream recovery (currently at best 30%, new nanofiber technologies coupled with next generation of ligands…