Search results for " ion-exchange"
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Mechanistic Modeling of Preparative Ion-Exchange Chromatography
For example, for the commonly used ion-exchange chromatography, pH, ion concentration, gradient, and stationary phase can affect the recovery step as well as the resulting product quality.
For com…
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Asked and Answered: Your Questions on Ion-Exchange Chromatography
Check out what questions your fellow protein purifiers have related to ion-exchange chromatography.
The questions are organized in by chief concern, and include buffers, experimental design, L…
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Process Chromatography Selection for Downstream Processing Applications
For these molecules, chromatographic techniques such as ion-exchange, hydrophobic interaction chromatography, and multimodal resins are common. Affinity resins like Capto L (antigen-binding fragments …
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Multi-product high-resolution monoclonal antibody charge variant separations by pH gradient ion-exchange chromatography. Anal Chem. 2009;81:8846–57.
17. Salas-Solano O, Gennaro L, Felten C. Optimi…
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CMOs Continue to Improve Overall Biomanufacturing Performance
Many are also using or evaluating a number of technologies, including:
• Membrane-based filtration technologies
• Ion-exchange membrane technologies
• Ion-exchange technologi…
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Evolution of the Monoclonal Antibody Purification Platform
Use of multimodal chromatography
Recently, mixed-mode, ion-exchange ligands with enhanced binding strength for aggregates through the addition of hydrophobic functionality have been brought to mar…
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Trends in Downstream Bioprocessing
Downstream processing involves the recovery and purification of protein therapeutics for biopharmaceuticals, vaccines, and other biologics. It involves the removal of insoluble cell debris and parti…
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How Can A Chromatography Bead Free Up Your Time?
Have you ever considered how a resin bead in your chromatography column can affect your ion exchange (IEX) chromatography run time?
Read this blog and learn how you can optimize the time spen…
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Impurity Testing of Biologic Drug Products
… of cellular origin via techniques such as SEC–HPLC, hydrophobic interaction chromatography (HIC), ion-exchange HPLC, and isoelectric focusing (IEF) capillary electrophoresis. Process-related impurit…
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Process Chromatography: Continuous Optimization
Several interaction modes
There are four major chromatographic techniques employed to purify biologic drug substances: affinity, ion-exchange (IEX), mixed- or multi-mode, and hydrophobic-interaction…