Search results for " capture" in Articles / App Notes
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
These principal components are arranged such that the first principal component (PC1) captures the maximum possible variance from the data set (equivalent to the line of best fit though the data set).…
Article
Continued Process Verification for Biopharma Manufacturing
One of the main struggles to resolve when implementing CPV will be to determine the types and quantity of data to be captured and analyzed. CPV can err on the side of providing too little data to the …
Article
Applying GMPs to the BioPharma Supply Chain
Deep data capture and analysis
Suppliers must have robust systems and tools for collecting data in their production processes and the causes of variation. They must be open to sharing these data w…
Article
A Platform Approach for the Purification of Domain Antibodies (Dabs)
First, a capture step using Capto™ L was used to reduce E. coli host cell proteins (ECP) and endotoxin levels.
Next, a polishing step using Capto MMC ImpRes further reduced ECP and endotoxin l…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
Protein A chromatography was used in the initial capture step, where the antibody in cell culture broth was loaded at neutral pH, washed with various solutions, and eluted at a lower pH value. The elu…
Article
Optimizing KiH bispecific antibody chromatography steps
This web article describes how Bioprocessing Technology Institute (BTI), Singapore together with scientists at Cytiva developed an optimized capture step for two closely related knob-into-hole (KiH) b…
Article
Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
The first method is using nonaffinity chromatography steps (AIEX + heat treatment + HIC), while the second method is using the novel affinity Cytiva™ Protein Select™ resin for the capture step, follow…
Article
Advances in Sterilization Technologies for Overcoming Viral-Vector Manufacturing Challenges
“For a 50-L bioreactor run, the intermediate downstream processing volumes can already be below 2 L after the capture steps, but single-use mixers currently on the market start at 50 L,” she explains.…
Article
Webinar: Intensifying downstream bioprocessing with continuous technologies
We will discuss:
• Intensification of capture chromatography and the benefits of coupling with viral inactivation
• How a single flow through chromatography step can simplify polishing
• The …
Article
Life cycle assessment of the protein A process in mAb manufacturing
Here we present a cradle-to-grave LCA for protein A chromatography resins in the capture step of mAb manufacturing. The focus is on the resin use phase, although resin manufacturing, packaging, distri…